Paper on improved antibody tag in Molecular Pharmaceutics

Developing an antibody tag with improved pharmacokinetics and stability allowed Tagworks to achieve increased tumor uptake of a radiolabeled pretargeting probe. Even though this adapted tag exhibited a slight loss in reactivity, its use in a antibody (CC49-TCO) conjugate demonstrated a longer clearance half-life, improved tumor accumulation and increased in vivo stability. The overall result was a 50% increase in tumor uptake as well as increased tumor/non-tumor ratios of the radiolabeled tetrazine probe. Read the Tagworks paper “Trans-cyclooctene tag with improved properties for tumor pretargeting with the Diels-Alder reaction” in Mol. Pharmaceutics 2014, 11(9). doi:10.1021/mp500725a

Current Opinion in Chemical Biology features Tagworks review

Approaches that use in vivo chemistry to enable non-invasive molecular imaging and therapy are much sought after. Taking the recent achievements in pretargeted radioimmuno-imaging and -therapy in mice as a starting point, the Tagworks team discuss how the application scope of this approach can eventually be extended towards humans. Read “Pretargeted imaging using bioorthogonal chemistry in mice” in Current Opinion in Chemical Biology 2014, 21:161-169. doi:10.1016/j.cbpa.2014.07.023